Asian Journal of Research in Chemistry

Brijen Vaghela ¹, Gunjansinh Parmar ², Shailesh Shah ², Dinesh Shah ²

Affiliations
1 Department of Pharmaceutical Analysis, Maliba Pharmacy College, Bardoli, Gujarat, IN
2 Depatment of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, IN

Abstract

The use of first order derivative spectrophotometry allows simultaneous determination of levosulpiride and esomeprazole in fixed dose combination products. The wavelengths 275 nm and 291.5 nm of first derivative spectrum were selected for the estimation of levosulpiride and esomeprazole, respectively without mutual interference. The method was linear in the concentration range 25-125 μg/ml and 5-25 μg/ml for levosulpiride and esomeprazole, respectively. The method was validated in terms of accuracy and precision. The result of the formulation analysis shows that the proposed method can be successfully used for the simultaneous estimation of both the drugs in their combined capsule dosage form.

Keywords

Levosulpiride, Esomeprazole, Ultra-violet spectrophotometry, Derivative spectrophotometry, Zero

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Khushbu Naik ¹, Gunjansinh Parmar ¹, Jalpesh Ahiya ¹, Kunjan Bodiwala ¹, Shailesh Shah ¹

Affiliations
1 Department of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, IN

Abstract

Derivative spectroscopy offers a useful approach for the analysis of drugs in multi-component mixtures. In this study a first-derivative spectroscopic method was used for the simultaneous determination of diclofenac and pantoprazole in combined capsule dosage form using the zero-crossing technique. The wavelengths 290.2 nm and 276.2 nm of first derivative spectrum were selected for the estimation of diclofenac and pantoprazole, respectively without mutual interference. The method was linear in the concentration range 15-75μg/ml and 5-25μg/ml for diclofenac and pantoprazole, respectively. Validation studies confirmed the accuracy and precision of the proposed method. The result of the formulation analysis shows that the proposed method can be successfully used for the simultaneous estimation of both the drugs in their combined capsule dosage form.

Keywords

Diclofenac, Pantoprazole, Simultaneous Determination, Derivative Spectrophotometry, Zero Crossing Point

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Nilesh Patel ¹, Shailesh Shah ², Ishwarsinh Rathod ³, Dhaval Patel ⁴, Biraju Patel ⁴

Affiliations
1 Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360005, Gujarat, IN
2 Department of Quality Assurance, Maliba Pharmacy College, Bardoli, Gujarat, IN
3 Department of Quality Assurance, L.M. College of Pharmacy, Ahmedabad-380009, Gujarat, IN
4 Veerayatan Institute of Pharmacy, Mandvi-370460, Gujarat, IN

Abstract

A simple, specific and precise HPTLC method was developed and validated for estimation of aceclofenac in photodegraded sample. The method involves separation of sample and photodegradation product on TLC aluminum sheets pre-coated with silica gel 60 F₂₅₄ plate using a mixture of Toluene:Ethyl acetate:Glacial acetic acid:Methanol (6.00:1.00:0.20:0.15 v/v/v/v) as the mobile phase. Detection of the spot was carried out at 284 nm in absorbance/reflectance mode. The retardation factor (R_f) for aceclofenac was found to be 0.35±0.03. The linearity range was found to be 50-150 ng/spot with r²=0.9931. The validated method was applied for determination of photodegradation kinetic study. Photodegradation reaction at various experimental conditions follows zero order kinetic and rate constant (K) was determined to be 0.4855 ng μl^-1 min^-1. T_1/2 and T_90% was found to be 116.78 min^-1 and 23.35 min^-1 respectively.

Keywords

Photodegradation Kinetics, Aceclofenac, HPTLC, Photostability.

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